Mandate

  • Standardization of clonal propagation protocol using biotechnological applications of tree & medicinal plant species.
  • Germplasm evaluation of medicinal plants through chemoprofiling.
  • Cryopreservation of rare, endangered and threatened medicinal plants.
  • Genetic diversity assessment of different species using molecular marker techniques.
  • Species specific identification through molecular marker technique for plant.

Institutional Staff

Name Dr. S.K. Tiwari
Qualification Scientist MSc - 1985 (Botany); M. Phil 1986 (Micology); PhD -1992 (Life Science Plant Tissue Culture)
Email address This email address is being protected from spambots. You need JavaScript enabled to view it.
Contact number 917566372511
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Project Staff

 

Research

Ongoing projects

Title of the project Name of PI Co-PI Source of funding Sanctioned amount (Rs) Date of initiation Due date of completion
Identification of potential pockets and selection of candidate plus trees of Bija and standardization of its clonal propagation technique. Dr. S.K. Tiwari Dr. A.K. Sharma PCCF (R/E and Lok Vaniki) MP Bhopal 11.20 lakhs Dec. 2019 Dec. 2020

 

Completed projects

Title of the project Name of PI Objectives Funding Date of completion
Genetic diversity assessment using molecular characterization, chemoprofiling, standardization of micropropagation and cryopreservation protocol of four RET species. (Berberis aristata, Swertia angustifolia, Embelia tsjeriam-cottam, Saraca asoka) Dr. S.K. Tiwari

1. To collect wild germplasm of targeted species.

2. To standardize the chemoprofiling technique using HPLC for the quantitative determination of active alkaloid.

3. To assess the genetic diversity through molecular markers for the identification of genetically diversified populations and genotypes.

4. To multiply chemically rich and genetically diversified population /genotypes for the production of elite planting material.

5. To develop appropriate cryopreservation technique for long term conservation of elite planting material.

6. To prepare the technical manual for the extension of evolve technologies.
NMPB, New Delhi June 2019
Quantitative determination of bio-active compounds of highly threatened medicinal plant species (Butea superba, Corallocarpus epigeous Alectra chitrakutensis) through chemoprofiling and standardization of propagation techniques using biotechnological interventions for their conservation. Dr. S.K. Tiwari

1. Collection of germplasm of medicinal plants of 3 categories of viz. critically endangered, endangered and near threat from different forest areas of M.P.

2. Standardization of   HPLC techniques through chemical profiling (chemoprofiling) for quantitative determination, estimation of bioactive compound (alkaloid) and to constitute a chemical fingerprint library.

3. Multiplication of identified germplasm by standardizing micro and macro propagation protocol in order to produce approximately 200 plants of each species.  

4. Standardization of cryopreservation protocol for long term conservation of targeted species.

5. Obtain the patenting for international recognition.

6. Preparation of technical manual for the technology studies on the targeted species.
APCCF (R/E and Lok Vaniki), MP. Bhopal. May 2019
Forensic DNA profiling and timber tracing for origin of wood with special reference to Tectona grandis (Teak) and Pterocarpus marsupium (Beeja). Dr. S.K. Tiwari

1. To strengthen Forest Genetics Division by establishing forest forensic DNA profiling lab for timber tracing.

2. To identify proper mechanism for controlling illegal fellings of proposed forestry tree species by establishing forensic DNA profiling and timber tracing for origin of wood.

APCCF (R/E and Lok Vaniki), MP. Bhopal. March 2020
Monitoring and evaluation of tissue culture grown plants of Dendrocalamus asper  in different forest divisions of Madhya Pradesh Dr. S.K. Tiwari To collect the growth performance data of tissue culture grown plants of Dendrocalamus asper. Mission Director, M.P. State Bamboo Mission, Bhopal. Nov. 2019
Production of Dendrocalamus asper through tissue culture technique Dr. S.K. Tiwari   Mission Director, M.P. State Bamboo Mission, Bhopal. 2019
Clonal multiplication of Dendrocalamus asper (Thailand bamboo) through micropropagation technique Dr. S.K Tiwari   M.P. State Bamboo Mission, Bhopal  March 2018
Clonal multiplication of Dendrocalamus asper (Thailand bamboo) through   miropropagation technique Dr. S.K Tiwari 1. To multiply & produce Dendrocalamusasper plants using micropropagation technique.
2. To produce 3000 plants per year.
Addl. PCCF. R&E & Lokvaniki  Bhopal Dec. 2014
Establishment of an advanced laboratory for molecular characterization and chemoprofiling of Commiphora wightii plant Dr. S.K Tiwari
  1. Survey and collection of wide range of germplasm from wild and their maintenance in mist chamber/green house for further investigation.
  2. Chemoprofiling of collected germplasm for the quantitative determination of guggulsterone.
  3. Assessment of genetic diversity through molecular markers technology.
M.P. Biotechnology Council, Bhopal Oct.2014

 

Major Research Achievements

  • Selection and identification of plus trees of Teak and other tree species
  • Establishment and maintenance of CSO (Teak)
  • Provenance Trials and their maintenance
  • Establishment of Seed Production Area of teak
  • Standardize of macropropagation protocols through rooting of cuttings under mist conditions for 36 important tree and medicinal plant species
  • Standardize micropropagation protocols of 7 commercially important timber tree species and 16 medicinal and aromatic plant.
  • Standardize chemoprofiling techniques for quantitative determination of bioactive compounds of 14 commercially viable medicinal plant species.
  • Standardize cryopreservation protocol for long term conservation of rare, endangered and threatened species.
  • Wood forensic study using molecular marker for the identification of origin of wood.
  • Wild life forensic study using molecular marker for the identification of wild life animals.
  • Provided trainings and dissertation courses for under graduate, post graduate and M. Phil students in the field of plant biotechnology and plant tissue culture.
  • Consultancy in the field of plant tissue culture.

 

Regular activities

To educate and impart professional technical training programme on plant biotechnology such as molecular marker, DNA finger printing, gene mapping, DNA sequencing, cryopreservation, Basic and applied techniques of plant tissue and demonstration of various instruments through lab visit for graduate, post graduate, M.Phil students & other stakeholders.

Services

  • (a) Branch has offered services in the form of provided trainings and dissertation courses for under graduate, post graduate and M. Phil students in the field of plant biotechnology and plant tissue culture on chargeable basis.
  • (b) Provide Ph.D. degree in the field of Plant Biotechnology, Botany.
  • (b) Consultancy in the field of plant tissue culture & biotechnology as per the institute norms.

Research summaries

Abstracts of Completed Project

  • a. Clonal multiplication of Dendrocalamus asper (Thailand bamboo) through miropropagation technique.

    Findings- D.asper is propagated by using micropropagation technique through nodal explants. Excellent morphogenetic response in terms of shoot induction and shoot multiplication observed, when MS culture medium was supplemented with combination of 3.0mg/lt. BAP & IAA 0.1mg /lt. In vitro raised shoots harvested and separated for root induction, profuse root induction observed when the MS culture medium was supplemented with NAA 3.0mg/lt and IAA 1.0mg/lt. 6500 In vitro regenerated plantlets were shifted in 1:1:1 FYM for acclimatization and hardening and after hardening 70% plants were survived.

  • b. Establishment of an advanced laboratory for molecular characterization and chemoprofiling of Commiphora wightii plant

    Findings-1.Chemoprofiling: The percent concentration of Guggulsterone in the accessions collected from different geographical locations of Rajasthan, Gujrat and MP are presented below-

    • Rajasthan: ranges from 0.65 % to 2.32%.
    • Gujarat: ranges from 0.59 % to 2.22%.
    • MP: ranges from 0.54 % to 2.01%.

    Findings-2. Genetic diversity - Population which collected from Kiradu (historical guggle in India) and surrounding areas of Badmer District (Rajasthan) showed highest genetic diversity is concern.

Training

Subjects

Duration

15 Days 30 Days 2 Months 3 months 4 months    6  months

1. DNA Molecular marker technology

Nil

Nil

Rs.10000/-

Rs.15000/-

Rs.20000/-

 Rs.30000/-

2. Genome Sequencing

Nil

Nil

Nil

Rs.15000/-

Rs.20000/-

 Rs. 30000/-

3. Genotyping

Nil

Nil

Nil

Rs.15000/-

 Rs.18000/-

 Rs. 25000/-

4. Gene expression   profiling

Nil

Nil

Nil

Rs.15000/-

Rs.20000/-

 Rs.30000/-

5. Genome informatics

Nil

Nil

Rs.10000/-

Rs.18000/-

Rs.25000/-

Rs. 35, 000/-

6. DNA Isolation technique & Electrophoresis

Nil

5000

Nil

Nil

Nil

Nil

6. Plant propagation & Plant Tissue culture

Rs. 3000/

Rs. 5000/-

Rs. 8000/-

Rs. 13000/-

Rs. 15000/-

  Rs. 20000/

8. Chemo-profiling for quantitative determination of active alkaloids of commercially important medicinal plants to quantify the alkaloids percent through HPLC.

Rs. 3000/ Rs. 5000/

 Rs. 8000/

 Rs. 13000/

 Rs. 15000/

 Rs. 20000/

Rs. 3000/ Rs. 5000/

 

Term and conditions for training and dissertation

1. The Trainee/student should deposit registration fee Rs. 2000/- either by cash or by sending the DD in favour of Director, State Forest Research Institute, Jabalpur. The registration fee will be non-refundable.  2. Only Accommodation (Without Meals/foods) will be provided on payment basis @ Rs. 50/per student per day in our hostel.  3. This will be ensuring by your written commitment and for this trainee should submit payment in advance which will be non-refundable.   4. Batches of 15-20 Trainee /students for different duration.